孙士生

个人信息:Personal Information

教授
博士生导师

教师英文名称:Shisheng Sun

教师拼音名称:Sun Shisheng

所在单位:生命科学学院

办公地点:西北大学太白校区,新生命科学学院2层

职称:教授

学科:生物化学与分子生物学
细胞生物学
生物学其他专业

论文成果

当前位置: 中文主页 >> 论文成果

21. Shisheng Sun*, Hui Zhang. Identification and Validation of Atypical N-Glycosylation Sites. Analytical chemistry. 2015. 87 (24): 11948–11951.

点击次数:

影响因子:5.9

DOI码:10.1021/acs.analchem.5b03886.

摘要:It is well-known that N-linked glycans usually attach to asparagine residues in the N-X-S/T motifs of proteins. However, accumulating evidence indicates that N-glycosylation could also possibly occur at other atypical motifs. In this study, we tried to identify atypical N-glycosylation sites using our recently developed solid-phase extraction of the N-linked Glycans And Glycosite-containing peptides (NGAG) method. Peptides with deamidation sites at asparagine residues but lacking a typical asparagine-X-serine/threonine sequons (N-X-S/T, X is any amino acid except proline) motif were identified from deglycosylated peptide data as potentially atypical glycosite-containing peptides. These atypical glycosites were verified by the presence of glycans on their intact glycopeptides and further confirmed by specific inhibition of cells with an N-linked glycosylation inhibitor, tunicamycin. From this study, two atypical N-linked glycosylation sites with N-X-C and N-X-V motifs were identified and validated from an ovarian cancer cell line (OVCAR-3).

论文类型:期刊论文

学科门类:理学

一级学科:生物学

文献类型:J

是否译文:

收录刊物:SCI