孙士生，西北大学生命科学学院教授，博士生导师。国家海外高层次人才青年项目 和 陕西省百人计划青年项目获得者。

        主要从事糖蛋白质组学方法学、肿瘤标记物发现和疾病致病机理研究（目前主要集中在恶性肿瘤和不育症）。主要研究方向：1) 糖蛋白质组学新技术、新软件；2) 新的肿瘤标志物发现； 3) 糖蛋白的功能和肿瘤发病机制；4) 重要糖蛋白的功能研究；5) 糖基化与男性不育。主要研究成果包括：① 建立了更加全面系统分析N-糖蛋白质组的NGAG方法及其他分析方法。② 开发了可实现位点特异性糖链精细结构解析的新方法和新软件-StrucGP，实现了糖蛋白质组的精细化、高通量和自动化解析; ③ 将糖蛋白质组学方法应用于各种疾病研究，从蛋白糖基化层面推动疾病检测、致病机理和分子分型等方面的研究。以第一或通讯作者在Nature Biotechnology, Nature Methods, Nature Communications, Theranostics, Analytical Chemistry 等杂志发表SCI论文 60 余篇; 申请中美专利 9 项，获批 8 项。

       科研条件：1) 实验室拥有全套蛋白质组和糖蛋白质组学分析平台，其中包括OrbitrapFusion Lumos三合一 质谱仪，HPLC 1260 (Agilent)及相应的数据分析软件，如Proteome Discoverer，MaxQuant，Byonic, GPQuest，StrucGP 及其他自开发软件。 2) 具备分子生物学实验平台； 3) 与西安多家医院 和 计算机科研团队 建立了稳定合作。

代表性文章：

1. Jiechen Shen#, Li Jia#, Liuyi Dang#, Yuanjie Su#, Jie Zhang, Yintai Xu, Bojing Zhu, Zexuan Chen, Jingyu Wu, Rongxia Lan, Zhifang Hao, Chen Ma, Ting Zhao, Ni Gao, Jieyun Bai, Yuan Zhi, Jun Li, Junying Zhang, Shisheng Sun*. StrucGP: de novo structural sequencing of site-specific N-glycan on glycoproteins using a modularization strategy. Nature Methods. 2021, 18: 921-929. (通讯作者, IF = 47.99)

2. Li Jia, Jun Li, Pengfei Li, Didi Liu, Jing Li, Jiechen Shen, Bojing Zhu, Chen Ma, Ting Zhao, Rongxia Lan, Liuyi Dang, Wang Li, Shisheng Sun*. Site-specific glycoproteomic analysis revealing increased core-fucosylation on FOLR1 enhances folate uptake capacity of HCC cells to promote EMT. Theranostics. 2021, 11 (14): 6905-6921.  (通讯作者, IF = 11.6)

3. Jianbo Pan#, Yingwei Hu#, Shisheng Sun#, Lijun Chen, Michael Schnaubelt, David Clark, Minghui Ao, Zhen Zhang, Daniel Chan, Jiang Qian, Hui Zhang*. Glycoproteomics-based signatures for tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer. Nature Communications. 2020, 11 (1): 6139.  (共同第一作者, IF = 14.9)

4. Shisheng Sun, Punit Shah, Shadi Toghi Eshghi, Weiming Yang, Namita Trikannad, Shuang Yang, Lijun Chen, Paul Aiyetan, Naseruddin Höti, Zhen Zhang, Daniel W. Chan, Hui Zhang*. Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nature Biotechnology. 2016, 34: 84-88.  (第一作者, IF = 41.667)

5.  Hui Zhang#, Tao Liu#, Zhen Zhang#, Samuel H Payne#, Bai Zhang, Jason E McDermott, Jian-Ying Zhou, Vladislav A Petyuk, Li Chen, Debjit Ray, Shisheng Sun, Feng Yang, Lijun Chen, Jing Wang, Punit Shah, Seong Won Cha, Paul Aiyetan, Sunghee Woo, Yuan Tian, Marina A Gritsenko, Therese R Clauss, Caitlin Choi, Matthew E Monroe, Stefani Thomas, Song Nie, Chaochao Wu, Ronald J Moore, ,Kun-Hsing Yu, David L Tabb, David Fenyö, Vineet Bafna, Yue Wang, Henry Rodriguez, Emily S Boja, Tara Hiltke, Robert C Rivers, Lori Sokoll, Heng Zhu, Ie-Ming Shih, Akhilesh Pandey, Bing Zhang, Michael P Snyder, Douglas A Levine, Richard D Smith, Daniel W Chan*, Karin D Rodland*, and the CPTAC investigators. Integrated proteogenomic characterization of human high grade serous ovarian cancer. Cell. 2016, 166 (3): 755-765.

团队名称：Glycoproteomics team

团队介绍：Developing new methods for glycoproteomic studies as well as applications in various biomedical samples mainly for new biomarker discovery and disease pathogenesis researches.