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An ICT-based fluorescent probe guided by theoretical calculation for selectively mapping endogenous GSH in living cells.
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影响因子：5.9
DOI码：10.1021/acs.analchem.5b01679.
摘要：Protein glycosylation is one of the most important protein modifications. Glycosylation site occupancy alteration has been implicated in human diseases and cancers. However, current glycoproteomic methods focus on the identification and quantification of glycosylated peptides and glycosylation sites but not glycosylation occupancy or glycoform stoichiometry. Here we describe a method for large-scale determination of the absolute glycosylation stoichiometry using three independent relative ratios. Using this method, we determined 117 absolute N-glycosylation occupancies in OVCAR-3 cells. Finally, we investigated the possible functions and the determinants for partial glycosylation.
论文类型：期刊论文
学科门类：理学
一级学科：生物学
文献类型：J
是否译文：否
收录刊物：SCI