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33. Shisheng Sun*, Yingwei Hu, Li Jia, Shadi Toghi Eshghi, Yang Liu, Punit Shah, Hui Zhang*. Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical N-Glycosylation Sites on Albumin and α-1B-Glycoprotein. Analytical chemistry. 2018, 90 (10): 6292–6299.
点击次数：
影响因子：6.35
DOI码：10.1021/acs.analchem.8b01051.
摘要：Most serum proteins are N-linked glycosylated, and therefore the glycoproteomic profiling of serum is essential for characterization of serum proteins. In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software. Our data indicated that half of identified N-glycosites were modified by at least two glycans, with a majority of them being sialylated. Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans. In addition, two novel atypical glycosites (with N-X-V motif) were identified and validated from albumin and α-1B-glycoprotein. The widespread presence of these two glycosites among individuals was further confirmed by individual serum analyses.
论文类型：期刊论文
学科门类：理学
一级学科：生物学
文献类型：J
是否译文：否
收录刊物：SCI
上一条：34. Yingchao Xue, Xiping Zhan, Shisheng Sun, Senthilkumar S Karuppagounder, Shuli Xia, Valina L Dawson, Ted M Dawson, John Laterra, Jianmin Zhang, Mingyao Ying*. Synthetic mRNAs drive highly efficient iPS cell differentiation to dopaminergic neurons. Stem Cells Translational Medicine. 2019, 8(2): 112-123.
下一条：32. Ganglong Yang, Yingwei Hu, Shisheng Sun, Chuanzi Ouyang, Weiming Yang, Qiong Wang, Michael Betenbaugh, Hui Zhang*. Comprehensive glycoproteomic analysis of Chinese hamster ovary cells. Analytical Chemistry. 2018, 90(24): 14294-14302.