37. Shisheng Sun*, Yingwei Hu, Minghui Ao, Punit Shah, Jing Chen, Weiming Yang, Xingwang Jia, Yuan Tian, Stefani Thomas, Hui Zhang*. N-GlycositeAtlas: a database resource for mass spectrometry-based human N-linked glycoprotein and glycosylation site mapping. Clinical Proteomics. 2019, 16 (1): 1-11.
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影响因子:2.568
DOI码:10.1186/s12014-019-9254-0.
摘要:Background: N-linked glycoprotein is a highly interesting class of proteins for clinical and biological research. The large-scale characterization of N-linked glycoproteins accomplished by mass spectrometry-based glycoproteomics has provided valuable insights into the interdependence of glycoprotein structure and protein function. However, these studies focused mainly on the analysis of specific sample type, and lack the integration of glycoproteomic data from different tissues, body fluids or cell types. Methods: In this study, we collected the human glycosite-containing peptides identified through their de-glycosylated forms by mass spectrometry from over 100 publications and unpublished datasets generated from our laboratory. A database resource termed N-GlycositeAtlas was created and further used for the distribution analyses of glycoproteins among different human cells, tissues and body fluids. Finally, a web interface of N-GlycositeAtlas was created to maximize the utility and value of the database. Results: The N-GlycositeAtlas database contains more than 30,000 glycosite-containing peptides (representing > 14,000 N-glycosylation sites) from more than 7200 N-glycoproteins from different biological sources including human-derived tissues, body fluids and cell lines from over 100 studies. Conclusions: The entire human N-glycoproteome database as well as 22 sub-databases associated with individual tissues or body fluids can be downloaded from the N-GlycositeAtlas website at http://nglycositeatlas.biomarkercenter.org.
论文类型:期刊论文
学科门类:理学
一级学科:生物学
文献类型:J
是否译文:否
收录刊物:SCI
上一条:38. Jian Shu, Liuyi Dang, Dandan Zhang, Punit Shah, Lijun Chen, Hui Zhang, Shisheng Sun*. Dynamic analysis of proteomic alterations in response to N‐linked glycosylation inhibition in a drug‐resistant ovarian carcinoma cell line. The FEBS journal. 286, 8: 1594-1605.
下一条:36. Bojing Zhu, Jiechen Shen, Ting Zhao, Haihai Jiang, Tianran Ma, Jie Zhang, Liuyi Dang, Ni Gao, Yingwei Hu, Yi Shi*, Shisheng Sun*. Intact glycopeptide analysis of influenza A/H1N1/09 neuraminidase revealing the effects of host and glycosite location on site‐specific glycan structures. Proteomics. 2019, 1800202.