39. Liuyi Dang, Jiechen Shen, Ting Zhao, Fei Zhao, Li Jia, Bojing Zhu, Chen Ma, Danqian Chen, Yingyong Zhao, Shisheng Sun*. Recognition of Bisecting N-Glycans on Intact Glycopeptides by Two Characteristic Ions in Tandem Mass Spectra. Analytical chemistry. 2019, 91(9): 5478-5482.
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影响因子:6.785
DOI码:10.1021/acs.analchem.8b05639.
摘要:Bisecting N-glycan represents one of the most important modifications to the N-glycan core, and it is involved in various biological processes. Despite many studies on the biological roles of bisecting N-glycans, current approaches for bisecting N-glycan analysis mainly rely on the use of the lectin PHA-E, which are of low specificity and sensitivity. Here, we describe a straightforward method for the recognition of bisecting N-glycans on intact glycopeptides using two characteristic Y ions [peptide+HexNAc3Hex1] and [peptide+HexNAc3Hex1Fuc1] in low energy fragmented MS/MS spectra under higher energy collisional dissociation (HCD) mode. The critical aspect of the method is the combination use of low energy HCD fragmentation and intact glycopeptide analysis. With samples from rat renal tissues, we determined the optimal fragmentation energies and analyzed the influence of core fucosylation on the intensity of the [peptide+HexNAc3Hex1] ion. Using the method, we identified 183 intact glycopeptides with bisecting N-glycans and investigated the primary bisecting N-glycan structures and the possible biological roles of these identified proteins.
论文类型:期刊论文
学科门类:理学
一级学科:生物学
文献类型:J
是否译文:否
收录刊物:SCI
上一条:40. Liuyi Dang, Li Jia, Yuan Zhi, Pengfei Li, Ting Zhao, Bojing Zhu, Rongxia Lan, Yingwei Hu, Hui Zhang, Shisheng Sun*. Mapping human N-linked glycoproteins and glycosylation sites using mass spectrometry. Trends in Analytical Chemistry. 2019. 114: 143-150.
下一条:38. Jian Shu, Liuyi Dang, Dandan Zhang, Punit Shah, Lijun Chen, Hui Zhang, Shisheng Sun*. Dynamic analysis of proteomic alterations in response to N‐linked glycosylation inhibition in a drug‐resistant ovarian carcinoma cell line. The FEBS journal. 286, 8: 1594-1605.