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影响因子：5.477

DOI码：10.1080/15476286.2018.1517014

发表刊物：RNA Biology

关键字：Malaria; RNA exosome; antisense RNA; Dis3 knock out; cryptic RNA

摘要：parasite Plasmodium falciparum. RNA-processing factors, however, remain poorly characterized in this pathogen. Here, we purified the multiprotein RNA exosome complex of malaria parasites by affinity chromatography, using HA-tagged PfRrp4 and PfDis3 as the ligands. Seven distinct core exosome subunits (PfRrp41, PfMtr3, PfRrp42, PfRrp45, PfRrp4, PfRrp40, PfCsl4) and two exoribonuclease proteins PfRrp6 and PfDis3 are identified by mass spectrometry. Western blot analysis detects Dis3 and Rrp4 predominantly in the cytoplasmic fraction during asexual blood stage development. An inducible gene knock out of the PfDis3 subunit reveals the upregulation of structural and coding RNA, but the vast majority belongs to antisense RNA. Furthermore, we detect numerous types of cryptic unstable transcripts (CUTs) linked to virulence gene families including antisense RNA in the rif gene family. Our work highlights the limitations of steady-state RNA analysis to predict transcriptional activity and link the RNA surveillance machinery directly with post-transcriptional control and gene expression in malaria parasites.

论文类型：期刊论文

学科门类：医学

一级学科：基础医学

卷号：15

期号：9

页面范围：1206–1214

ISSN号：1547-6286

是否译文：否

收录刊物：SCI

发布期刊链接：https://doi.org/10.1080/15476286.2018.1517014