点击次数：

影响因子：4.088

DOI码：10.1016/j.jprot.2012.11.020

发表刊物：J Proteomics

关键字：Plasmodium falciparumAntibody responseMerozoiteAntigenProtein array

摘要：With the completion of the functional genome, merozoite proteome and stage-specific transcriptomes of the intraerythrocytic developmental cycle of Plasmoidum falciparum, the development of new vaccine candidates targeting Plasmodium merozoites is now possible. Here we report using protein array technology to detect antibody responses to Plasmodium merozoite proteins by screening the serum of Plasmodium-exposed individuals. A total of 138 genes encoding P. falciparum merozoite proteins were cloned using the In-Fusion cloning method and expressed using a wheat germ cell-free system (WGCF). These proteins were then screened with serum from Plasmodium-exposed individuals and unexposed subjects using protein arrays. A total of 30 highly immunoreactive merozoite antigens were identified (21.7% of 138 target proteins), including 10 well-characterized blood-stage vaccine candidates for P. falciparum. In addition, we report for the first time 7 proteins (MSP3.5, MRSP2, ETRAMP11.2, ETRAMP14.1 and RALP1, and two hypothetical proteins PFA0210c and PF14_0572) as being immunologically reactive. These novel Plasmodium merozoite antigens may be potential vaccine candidates for blood-stage malaria, and warrant further study.

论文类型：期刊论文

学科门类：医学

一级学科：基础医学

卷号：78

页面范围：148-58

是否译文：否

收录刊物：SCI

发布期刊链接：https://doi.org/10.1016/j.jprot.2012.11.020