Professor
Supervisor of Doctorate Candidates
Supervisor of Master's Candidates
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Impact Factor5.9
DOI number:10.1021/acs.analchem.5b03886.
Abstract:It is well-known that N-linked glycans usually attach to asparagine residues in the N-X-S/T motifs of proteins. However, accumulating evidence indicates that N-glycosylation could also possibly occur at other atypical motifs. In this study, we tried to identify atypical N-glycosylation sites using our recently developed solid-phase extraction of the N-linked Glycans And Glycosite-containing peptides (NGAG) method. Peptides with deamidation sites at asparagine residues but lacking a typical asparagine-X-serine/threonine sequons (N-X-S/T, X is any amino acid except proline) motif were identified from deglycosylated peptide data as potentially atypical glycosite-containing peptides. These atypical glycosites were verified by the presence of glycans on their intact glycopeptides and further confirmed by specific inhibition of cells with an N-linked glycosylation inhibitor, tunicamycin. From this study, two atypical N-linked glycosylation sites with N-X-C and N-X-V motifs were identified and validated from an ovarian cancer cell line (OVCAR-3).
Indexed by:Journal paper
Discipline:Natural Science
First-Level Discipline:Biology
Document Type:J
Translation or Not:no
Date of Publication:2015-12-15
Included Journals:SCI
Pre One:22. Xingwang Jia, Jing Chen, Shisheng Sun, Weiming Yang, Shuang Yang, Punit Shah, Naseruddin Hoti, Bob Veltri, Hui Zhang. Detection of Aggressive Prostate Cancer‐Associated Glycoproteins in Urine using Glycoproteomics and Mass Spectrometry. Proteomics. 2016, 16: 2989–2996.
Next One:20. Shisheng Sun, Hui Zhang*. Large-scale measurement of absolute protein glycosylation stoichiometry. Analytical chemistry. 2015. 87 (13): 6479-6482.
