Shisheng Sun

Professor

College of Life Sciences

Northwest University

229 Taibai North Road, Xi’an, Shaanxi Province 710069, P.R. China.

Email: suns@nwu.edu.cn

Tel: 86-18792738699

Research Interests 

Developing new methods for comprehensive glycoproteomic studies, as well as applying glycoproteomic methods into various biomedical samples with different physiological and pathological conditions for a systems biology-level understanding of the consequences of perturbed biological processes. 

During the last few years, he mainly developed: 1) StrucGP, a modularization strategy together with an algorithm, for de novo interpretation of N-glycan structures on intact glycopeptides using tandem mass spectrometry (Nature Methods, 2021); StrucGP can discriminate glycan isoforms on intact glycopeptides and facilitate the identification of rare/new glycan structures in different biomedical samples. 2) the NGAG method and related tools for comprehensive characterization of N-glycoproteome, with which information of N-glycans, glycoproteins, and glycosites, as well as site-specific glycosylation and occupancy can be obtained simultaneously (Nature Biotechnology, 2016); 3) new methods for characterizing site-specific N-glycans with certain structures (Analytical Chemistry, 2019); and 4) Real-world application of glycoproteomics and the successful discovery of glycosylation alterations in biomedical samples including ovarian cancers (Cell, 2016; Nature Communications, 2020) and liver cancer (Theranostics, 2021). These applications are crucial for the understanding the biological roles of glycosylation and could help us to explore the possibilities of glycans as diagnostic biomarkers and therapeutic targets.

Selected Publications：

1. Jiechen Shen#, Li Jia#, Liuyi Dang#, Yuanjie Su#, Jie Zhang, Yintai Xu, Bojing Zhu, Zexuan Chen, Jingyu Wu, Rongxia Lan, Zhifang Hao, Chen Ma, Ting Zhao, Ni Gao, Jieyun Bai, Yuan Zhi, Jun Li, Junying Zhang, Shisheng Sun*. StrucGP: de novo structural sequencing of site-specific N-glycan on glycoproteins using a modularization strategy. Nature Methods. 2021, 18: 921-929.

2. Li Jia, Jun Li, Pengfei Li, Didi Liu, Jing Li, Jiechen Shen, Bojing Zhu, Chen Ma, Ting Zhao, Rongxia Lan, Liuyi Dang, Wang Li, Shisheng Sun*. Site-specific glycoproteomic analysis revealing increased core-fucosylation on FOLR1 enhances folate uptake capacity of HCC cells to promote EMT. Theranostics. 2021, 11 (14): 6905-6921.

3. Jianbo Pan#, Yingwei Hu#, Shisheng Sun#, Lijun Chen, Michael Schnaubelt, David Clark, Minghui Ao, Zhen Zhang, Daniel Chan, Jiang Qian, Hui Zhang*. Glycoproteomics-based signatures for tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer. Nature Communications. 2020, 11 (1): 6139.

4. Shisheng Sun, Punit Shah, Shadi Toghi Eshghi, Weiming Yang, Namita Trikannad, Shuang Yang, Lijun Chen, Paul Aiyetan, Naseruddin Höti, Zhen Zhang, Daniel W. Chan, Hui Zhang*. Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nature Biotechnology. 2016, 34: 84-88.

5. Hui Zhang#, Tao Liu#, Zhen Zhang#, Samuel H Payne#, Bai Zhang, Jason E McDermott, Jian-Ying Zhou, Vladislav A Petyuk, Li Chen, Debjit Ray, Shisheng Sun, Feng Yang, Lijun Chen, Jing Wang, Punit Shah, Seong Won Cha, Paul Aiyetan, Sunghee Woo, Yuan Tian, Marina A Gritsenko, Therese R Clauss, Caitlin Choi, Matthew E Monroe, Stefani Thomas, Song Nie, Chaochao Wu, Ronald J Moore, ,Kun-Hsing Yu, David L Tabb, David Fenyö, Vineet Bafna, Yue Wang, Henry Rodriguez, Emily S Boja, Tara Hiltke, Robert C Rivers, Lori Sokoll, Heng Zhu, Ie-Ming Shih, Akhilesh Pandey, Bing Zhang, Michael P Snyder, Douglas A Levine, Richard D Smith, Daniel W Chan*, Karin D Rodland*, and the CPTAC investigators. Integrated proteogenomic characterization of human high grade serous ovarian cancer. Cell. 2016, 166 (3): 755-765.