Professor
Supervisor of Doctorate Candidates
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Impact Factor6.35
DOI number:10.1021/acs.analchem.8b01051.
Abstract:Most serum proteins are N-linked glycosylated, and therefore the glycoproteomic profiling of serum is essential for characterization of serum proteins. In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software. Our data indicated that half of identified N-glycosites were modified by at least two glycans, with a majority of them being sialylated. Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans. In addition, two novel atypical glycosites (with N-X-V motif) were identified and validated from albumin and α-1B-glycoprotein. The widespread presence of these two glycosites among individuals was further confirmed by individual serum analyses.
Indexed by:Journal paper
Discipline:Natural Science
First-Level Discipline:Biology
Document Type:J
Translation or Not:no
Date of Publication:2018-05-15
Included Journals:SCI
Pre One:49. Jianbo Pan#, Yingwei Hu#, Shisheng Sun#, Lijun Chen, Michael Schnaubelt, David Clark, Minghui Ao, Zhen Zhang, Daniel Chan, Jiang Qian, Hui Zhang*. Glycoproteomics-based signatures for tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer. Nature communications. 2020, 11 (1): 6139.
Next One:47. Ting Zhao, Li Jia, Jun Li, Chen Ma, Jingyu Wu, Jiechen Shen, Liuyi Dang, Bojing Zhu, Pengfei Li, Yuan Zhi, Rongxia Lan, Yintai Xu, Zhifang Hao, Yichao Chai, Qingshan Li, Liangshuo Hu, Shisheng Sun*. Heterogeneities of site-specific N-glycosylation in HCC tumors with low and high AFP concentrations. Frontiers in Oncology. 2020, 10: 496.
