Professor
Supervisor of Doctorate Candidates
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DOI number:10.1111/febs.14811.
Key Words:ER stress; cellular response; glycosylation; proteome; tunicamycin.
Abstract:Glycosylation inhibition can improve the efficacy of antitumor drugs and enhance the apoptosis of cancer cells, thus holding great potential for cancer treatment. Inhibition of N-glycosylation induces endoplasmic reticulum (ER) stress and the unfolded protein response (UPR), and eventually triggers ER stress-related apoptosis. Unfortunately, the detailed timeline of these cell responses and protein expression alterations related to N-glycosylation inhibition is not explicit yet, and the pathways involved in different stages of N-glycosylation inhibition still need to be characterized. In this study, the dynamic proteome alterations related to N-glycosylation inhibition were investigated by further analyzing our previously published quantitative proteomics data from tunicamycin (TM)-treated ovarian carcinoma (OVCAR-3) cells. The results revealed that N-glycosylation inhibition not only directly affects the expression of glycosylated proteins but also alters an extended scale of proteins. Functional annotation of these altered proteins demonstrated that proteins related to ER stress start changing within 6 h, followed by UPR within 24 h, and eventually ER stress-related apoptosis is triggered after 48 h, indicating the conversion of cellular response from positive to negative. The dynamic proteome data presented here provide important information for better understanding of the significance of N-glycosylation to cell survival and TM-related cancer treatment.
Indexed by:Journal paper
Discipline:Natural Science
First-Level Discipline:Biology
Document Type:J
Translation or Not:no
Date of Publication:2019-04-01
Included Journals:SCI
Pre One:39. Liuyi Dang, Jiechen Shen, Ting Zhao, Fei Zhao, Li Jia, Bojing Zhu, Chen Ma, Danqian Chen, Yingyong Zhao, Shisheng Sun*. Recognition of Bisecting N-Glycans on Intact Glycopeptides by Two Characteristic Ions in Tandem Mass Spectra. Analytical chemistry. 2019, 91(9): 5478-5482.
Next One:37. Shisheng Sun*, Yingwei Hu, Minghui Ao, Punit Shah, Jing Chen, Weiming Yang, Xingwang Jia, Yuan Tian, Stefani Thomas, Hui Zhang*. N-GlycositeAtlas: a database resource for mass spectrometry-based human N-linked glycoprotein and glycosylation site mapping. Clinical Proteomics. 2019, 16 (1): 1-11.
